Sci 115 microarray lab and quiz


  

Question 1 

  1. Most scientists who use DNA      microarrays achieve them 

  

from companies that majority consequence them   

 

from the government 

 

by making them in their labs 

 

by exploiting ambitious furrow   students 

4 apexs   

Question 2 

  1. "In this con-over, cancerous and      usual bark edifice specimens were excised after a suitableness a scalpel. Each bark specimen      was then placed into a specimen tube along after a suitableness solvent, oscillaten on a hurry      and centrifuged. When the specimen tubes came out of the centrifuge, there      was a top flake and a ground flake. For our resolves, we went on to use      ______________ for raise regularitying." 

  

only the top flake 

 

only the ground flake 

 

a comback of top and ground flakes 

 

none of the options listed 

4 apexs   

Question 3 

  1. The resolve of the hurry      instrument is to 

  

vigorously oscillate the edifice specimen in   the solvent so that it dissolves. 

 

spin the edifice/solvent comback   around to detached the solids 

 

motive the mRNA to unite to the pellets   

 

dispose of biohazardous throw-away safely 

3 apexs   

Question 4 

  1. "Once the RNA is unadorned      from the DNA, proteins and other materials, the solvent/RNA comback is run      through a column of all elaborates. Each elaborate has sundry lacking strands of      polyT RNA holding out of it. In this step:" 

  

"mRNA holds to the elaborates, suitableness   rRNA and tRNA run proper through the column" 

 

"tRNA holds to the elaborates, suitableness   mRNA and rRNA run proper through the column" 

 

"rRNA and tRNA hold to the   beads, suitableness mRNA runs proper through the column" 

 

none of the options listed 

4 apexs   

Question 5 

  1. "Once the mRNA is unadorned,      you constitute a DNA delineation of it using by adding polyT primers, labeled DNA      nucleotides, and an enzyme notorious as:" 

  

reverse counterfeitase 

 

tyrosine hydroxylase 

 

alcohol dehydrogenase 

 

ATP kinase 

4 apexs   

Question 6 

  1. A DNA delineation of a mRNA counterfeit is      notorious as 

  

complementary DNA (abbreviated cDNA) 

 

copied DNA (abbreviated cDNA) 

 

mitochondrial DNA (abbreviated mtDNA)   

 

recombinant DNA (abbreviated rDNA) 

3 apexs   

Question 7 

  1. Each disfigurement on the DNA microset-in-order in      embedded after a suitableness 

  

copies of DNA from one apex   gene 

 

copies of DNA from sundry contrariant   genes after a suitableness homogeneous functions 

 

copies of DNA from sundry contrariant   genes after a suitableness contrariant functions 

 

copies of mRNA from one or over genes   

4 apexs   

Question 8 

  1. "When we say that the cDNA      derived from our specimen has hybridized to a apex disfigurement on the set-in-order,      we medium that: " 

  

The cDNA from the specimen has gather to   the DNA on the microset-in-order at that apex. 

 

The cDNA from the specimen is heaped up   and cannot be trusted. 

 

The cDNA has been used to constitute an   mRNA delineation 

 

You scarcity to buy microarrays from   contrariant manufacturers and triangulate the results 

4 apexs   

Question 9 

  1. "In this apex      experiment, we used red-labeled DNA to regularity the specimen from      _____________ edifice and the fresh-labeled DNA to regularity the specimen from      ___________. " 

  

cancerous; usual 

 

normal; cancerous 

 

normal; usual 

 

cancerous; cancerous 

3 apexs   

Question 10 

  1. "After scanning the fresh      labeled areas and the red labeled areas, when we combine the two images,      the disfigurements that pomp up as yellow fit to " 

  

genes developed by twain usual or   cancerous bark cells. 

 

genes that were not developed at all   in either usual or cancerous bark cells 

 

"genes developed by cancerous,   but not usual bark cells" 

 

"genes developed by usual, but   not cancer bark cells. " 

4 apexs   

Question 11 

  1. "In interpreting the results      of this con-over, disfigurements on the microset-in-order that are red fit to:" 

  

genes that are acetous up by cancer 

 

genes that are acetous down by cancer 

 

genes that are sincere by cancer 

 

genes that aren t developed in usual   or cancerous cells 

4 apexs   

Question 12 

  1. "In interpreting the results      of this con-over, disfigurements on the the microset-in-order that are fresh fit to      genes that are " 

  

genes that are acetous down by cancer 

 

genes that are acetous up by cancer 

 

genes that are sincere by cancer 

 

genes that aren t developed in usual   or cancerous cells 

3 apexs   

Question 13 

  1. "When the DNA microset-in-order      con-over tells us that a comprehensive compute of genes own been acetous up (or acetous      down) by a conjecture, the most slight exposition is that " 

  

the acetous up genes are slight   controlled by a gene that has bybybypast bad 

 

all of these genes are genes that   own bybybypast bad themselves 

 

even equitable one back in any of   these unsupposable genes would own been competent to motive the conjecture 

 

the cytoplasm has too sundry permitted   radicals in it 

4 apexs   

Question 14 

  1. A gene pompn by the microset-in-order to      be developed is : 

  

"probably making protein, but a   protein look segregation would be scarcityed to perceive for sure" 

 

definitely making protein 

 

definitely not making protein 

 

probably making protein but there's   no way to perceive for sure 

4 apexs   

Question 15 

  1. "In the collision of this      technique to bark cancer, a gene that has bybybypast bad " 

  

"may or may not be signed at   all, but the overall mould of results can communicate main clues" 

 

can approximately frequently be signed via   DNA microarray