Sci 115 microarray lab and quiz


  

Question 1 

  1. Most scientists who use DNA      microarrays succeed them 

  

from companies that bulk profit them   

 

from the government 

 

by making them in their labs 

 

by exploiting ambitious disequalize   students 

4 objects   

Question 2 

  1. "In this con-over, cancerous and      ordinary husk structure cases were excised after a conjuncture a scalpel. Each husk case      was then placed into a case tube parallel after a conjuncture solvent, tremblen on a commotion      and centrifuged. When the case tubes came out of the centrifuge, there      was a top flake and a deep flake. For our objects, we went on to use      ______________ for prefer ruleing." 

  

only the top flake 

 

only the deep flake 

 

a adjustment of top and deep flakes 

 

none of the options listed 

4 objects   

Question 3 

  1. The object of the commotion      instrument is to 

  

vigorously tremble the structure case in   the solvent so that it dissolves. 

 

spin the structure/solvent adjustment   around to severed the solids 

 

suit the mRNA to fasten to the pellets   

 

dispose of biohazardous pine safely 

3 objects   

Question 4 

  1. "Once the RNA is unblended      from the DNA, proteins and other materials, the solvent/RNA adjustment is run      through a shaft of all con-overs. Each con-over has diverse blunt strands of      polyT RNA adhereing out of it. In this step:" 

  

"mRNA adheres to the con-overs, conjuncture   rRNA and tRNA run fit through the shaft" 

 

"tRNA adheres to the con-overs, conjuncture   mRNA and rRNA run fit through the shaft" 

 

"rRNA and tRNA adhere to the   beads, conjuncture mRNA runs fit through the shaft" 

 

none of the options listed 

4 objects   

Question 5 

  1. "Once the mRNA is unblended,      you mould a DNA representation of it using by adding polyT primers, labeled DNA      nucleotides, and an enzyme distinguishn as:" 

  

reverse facsimilease 

 

tyrosine hydroxylase 

 

alcohol dehydrogenase 

 

ATP kinase 

4 objects   

Question 6 

  1. A DNA representation of a mRNA facsimile is      known as 

  

complementary DNA (abbreviated cDNA) 

 

copied DNA (abbreviated cDNA) 

 

mitochondrial DNA (abbreviated mtDNA)   

 

recombinant DNA (abbreviated rDNA) 

3 objects   

Question 7 

  1. Each disfigurement on the DNA microdress in      embedded after a conjuncture 

  

copies of DNA from one detail   gene 

 

copies of DNA from diverse opposed   genes after a conjuncture congruous functions 

 

copies of DNA from diverse opposed   genes after a conjuncture opposed functions 

 

copies of mRNA from one or further genes   

4 objects   

Question 8 

  1. "When we say that the cDNA      derived from our case has hybridized to a detail disfigurement on the dress,      we medium that: " 

  

The cDNA from the case has store to   the DNA on the microdress at that object. 

 

The cDNA from the case is heaped up   and cannot be trusted. 

 

The cDNA has been used to mould an   mRNA representation 

 

You want to buy microarrays from   opposed manufacturers and triangulate the results 

4 objects   

Question 9 

  1. "In this detail      experiment, we used red-labeled DNA to rule the case from      _____________ structure and the unprepared-labeled DNA to rule the case from      ___________. " 

  

cancerous; ordinary 

 

normal; cancerous 

 

normal; ordinary 

 

cancerous; cancerous 

3 objects   

Question 10 

  1. "After scanning the unprepared      labeled areas and the red labeled areas, when we conjoin the two images,      the disfigurements that profession up as yellow harmonize to " 

  

genes developed by twain ordinary or   cancerous husk cells. 

 

genes that were not developed at all   in either ordinary or cancerous husk cells 

 

"genes developed by cancerous,   but not ordinary husk cells" 

 

"genes developed by ordinary, but   not cancer husk cells. " 

4 objects   

Question 11 

  1. "In interpreting the results      of this con-over, disfigurements on the microdress that are red harmonize to:" 

  

genes that are bitter up by cancer 

 

genes that are bitter down by cancer 

 

genes that are simple by cancer 

 

genes that aren t developed in ordinary   or cancerous cells 

4 objects   

Question 12 

  1. "In interpreting the results      of this con-over, disfigurements on the the microdress that are unprepared harmonize to      genes that are " 

  

genes that are bitter down by cancer 

 

genes that are bitter up by cancer 

 

genes that are simple by cancer 

 

genes that aren t developed in ordinary   or cancerous cells 

3 objects   

Question 13 

  1. "When the DNA microdress      con-over tells us that a bulky estimate of genes keep been bitter up (or bitter      down) by a assumption, the most likely exposition is that " 

  

the bitter up genes are likely   controlled by a gene that has past bad 

 

all of these genes are genes that   keep past bad themselves 

 

even normal one mutation in any of   these unsupposable genes would keep been ample to suit the assumption 

 

the cytoplasm has too sundry bountiful   radicals in it 

4 objects   

Question 14 

  1. A gene professionn by the microdress to      be developed is : 

  

"probably making protein, but a   protein countenance decomposition would be wanted to distinguish for sure" 

 

definitely making protein 

 

definitely not making protein 

 

probably making protein but there's   no way to distinguish for sure 

4 objects   

Question 15 

  1. "In the application of this      technique to husk cancer, a gene that has past bad " 

  

"may or may not be attested at   all, but the overall design of results can present influential clues" 

 

can approximately regularly be attested via   DNA microarray