Osmosis | Biology homework help


WEEK 3 EXPERIMENT ANSWER SHEET

Please comply to the Week 3 Experiment dropbox no later than Sunday midnight.

SUMMARY OF ACTIVITIES FOR WEEK 1 EXPERIMENT ASSIGNMENT

 

·         Experiment 3 Exertion 1 – Diffusion: Change-of-place of Solutes counter a Membrane

·         Experiment 3 Exercise 2 – Osmosis: Change-of-place of Impart counter a Membrane

 

 

Experiment 3 Exertion 1: Colliquation - Change-of-place of Solutes counter a Membrane

 

We conciliate be using dialysis tubing to counterfeit a semipercolable membrane. This tubing allows minute molecules (e.g., impart, ions, glucose) to by conjuncture preventing extensive molecules (e.g., macromolecules move proteins, starch, glycogen) from emotional counter.  Be fast you possess recognize aggravate the suggested illustrateative precedently starting this exertion and that you possess reviewed the subjoined animations:

McGraw-Hill. 2006. How Colliquation Works

https://highered.mcgraw-hill.com/sites/0072495855/student_view0/chapter2/animation__how_diffusion_works.html

McGraw-Hill. 2006. How Osmosis Works

https://highered.mcgraw-hill.com/sites/0072495855/student_view0/chapter2/animation__how_osmosis_works.html

 

 

Experimental Design

 

A.      The dialysis bag we conciliate use is percolable to impart and minute molecules (e.g., close than 500 g/mol) and impercolable to extensive molecules (e.g., further than 500 g/mol).

B.      The dialysis bag is employed delay a mixture of glucose (molecular importance = 180 g/mol) and protein (molecular importance = 10,000 g/mol) dissolved in impart. A minute subsample of the dialysis bag interruption is saved and conciliate be used in Step 4.

C.      The dialysis bag is then placed into a beaker of water. A minute subsample of beaker impart is besides saved and is to be used in Step 4 as well-mannered.

 

 

The closeness or insufficiency of glucose and protein conciliate be unshaken using indicators. Indicators fluctuate colors in the closeness fixed illustrateatives. The two tests that we’ll use are the Benedict’s test for uncompounded sugars (e.g., glucose) and theBiuret test for the closeness of proteins.

·         If glucose is give, the Benedict’s indicator conciliate turn green. If no glucose is give, the separation conciliate be blue.

·         If protein is give, the Biuret indicator conciliate turn violet.  If the separation remains clear, then no protein is give.

4.     The subsample of dialysis bag separation and the beaker impart are tested for the closeness of glucose and protein. SeeTable 1 under for the results.

5.     The dialysis bag is then left in the beaker of impart for 60 minutes.

6.     At the end of 60 minutes, the dialysis bag separation and the beaker impart are repeatedly tested for the closeness of glucose and protein. See Table 1 under for the results.

 

Table 1. Results of testing of the dialysis bag and beaker interruption at the commencement and end of the Experiment.

 

Test for Glucose

Test for Protein

 

Beginning

End

Beginning

End

Dialysis Bag

Green

Green

Violet

Violet

Beaker

Blue

Green

Clear

Clear

 

Questions

1.     Summarize the results in-reference-to the closeness (+) or insufficiency (-) of glucose and protein in the dialysis bag and beaker in Table 2 under (4 pts):

 

 

 

Table 2.

 

Glucose

Protein

 

Beginning

End

Beginning

End

Dialysis Bag

 

 

 

 

Beaker

 

 

 

 

 

2.     Explain the change-of-place or closing of change-of-place of protein and glucose counter the dialysis bag membrane (4 pts)

3.     Which separation, that in the bag or that in the beaker, is hypotonic compared delay the protein separation (2 pts)?

4.     What elements move the change-of-place of molecules counter a semipercolable membrane? Which element plays the principal role in biological systems (4 pts)?

 

5.     Briefly illustrate what free enravishment is and how it differs from byive enravishment, especially in conditions of attention gradients (4 pts).

 

 

 

 

Experiment 3 Exertion 2: Osmosis - The Change-of-place of Impart counter a Membrane

 

Before starting, let’s see what you understand encircling the conditions hypotonic, isotonic and hypertonic. Examine the diagrams under. Note that the minute fresh circles regive dissolved solutes move salt, glucose, and amino acids. You can presume that the subjoined room exclusive the solutes is water and that the tan area is INSIDE the cell.

 

Question

 

1.      Define each signal under in conditions of solute attention beyond compared to the within of the cell. You do not need to illustrate which tendency impart conciliate move (3 pts).

a.      Hypotonic -

 

b.      Isotonic - 

c.       Hypertonic -

 

Procedure

1.     Open the subjoined website to get started:

The Biology Place. No Date. Osmosis: Change-of-place of Impart counter Membranes

http://www.phschool.com/science/biology_place/biocoach/biomembrane1/osmosis.html

B.      Read aggravate the counsel giveed and then Click on 

C.      Then, Click on .  Recognize through the counsel giveed and be fast to click on Animate beneath the exemplification.

Questions

2.      What attention of salt is isotonic to carnal cells (1 pts)? 

 

 

3.      When cells are in isotonic separation, is there change-of-place of impart into or out of the cell?  If so, portray this change-of-place (3 pts).  

 

 

Procedure (continued)

4.     Click on  .

E.      Read through the counsel giveed and be fast to click on Animate beneath the exemplification. When recognizey, defense the subjoined topic.

 

 

Question

4.      Describe the net change-of-place of impart molecules when cells are placed in a hypotonic solution.  Explain why impart moves this way (3 pts).

 

 

 

Procedure (continued)

 

6.     Click on  

G.     Read through the counsel giveed and be fast to click on Animate beneath each of the exemplifications. Defense the subjoined topics. Your defenses should thicken the terminology used in the animations.

Questions

5.      What happens to an carnal cell when placed in a hypotonic solution (2 pts)?

 

 

6.      What happens to stock cells when placed in a hypotonic solution? What accounts for the distinction in outcomes among carnal cells and stock cells (3 pts)?

 

 

Procedure (continued)

8.     Click on  

I.        Then, Click on  .  Recognize through the counsel giveed and be fast to click on Animate beneath the exemplification. Defense the subjoined topic.

 

 

Question

7.      Describe the net change-of-place of impart molecules when cells are placed in a hypertonic solution.  Explain why impart moves this way (3 pts).

 

 

 

Procedure (continued)

10.  Click on  

K.      Read through the counsel giveed and be fast to click on Animate beneath the exemplification. Defense the subjoined topics.

Questions

8.    Compare and dissimilarity what happens to plant and animal cells when placed in a hypertonic separation. Be fast to use personal signalinology (4 pts).

 

 

9.    Based on what you versed in this exertion, illustrate why salt might effect a good-natured-natured deracinate killer (3 pts).

 

 

Week 3 Experiment Grading Rubric

 

Component

Expectation

Points

Experiment 3 Exertion 1

Interpretation of results and demonstrated discernment of colliquation (Table 2 and Questions 1-5).

18 pts

Experiment 3 Exertion 2

Demonstrates discernment of isotonic separations and no net change-of-place of impart (Questions 1-3).

7 pts

Demonstrates discernment of hypotonic separations and the change-of-place of impart (Questions 4-6).

8 pts

Demonstrates discernment of hypertonic separations and the change-of-place of impart (Questions 7-9).

10 pts

TOTAL

 

43 pts