Enzymes: temperature, ph, and specificity hands-on labs, inc.


0Enzymes: Temperature, pH, and Specificity
Hands-On Labs, Inc.  Version 42-0054-00-01
Lab Declaration Assistant                                                                                               This muniment is not meant to be a commute for a methodical laboratory declaration. The Lab Declaration Assistant is simply a resume of the test’s questions, diagrams if needed, and axioms tables that should be addressed in a methodical lab declaration. The fixed is to mature students’ fitness of lab declarations by providing this notification in an editable improve which can be sent to an tutor.
Exercise 1: Enzyme Specificity
Observations                                                                                                            
Data Table 1: Glucose Concentration
Wells Concentration of Glucose





 
Questions  

A.  What enumerates a person’s force to order lactose?
B.   Which of the wells showed a assured end for glucose? Explain the ends.
C.   Explain why testing for glucose is used to enumerate the vital-force of the enzyme lactase.
D.  Explain the testal provisions for the five irrelative wells.
 
Data Table 2: Presence of glucose in wells indicating lactase vital-force at manifold spheres
Well Time (min) Concentration of Glucose

a 5 
 10 
 15 
 20 
b 5 
 10 
 15 
 20 
c 5 
 10 
 15 
 20 
Exercise 2: Enzymes and Temperature
Observations  
 
Questions:  
A.  Graph the commodities of sphere on the vital-force of the enzyme lactase.
B.   What happens when an enzyme is boiled? Is this commodities alterable?
C.   Based on your test ends, what is the optimal sphere for lactase exercise?
D.  Explain what happens as far as the commoditiesiveness of the enzyme at the freezing sphere. Can this commodities be conquer when the sphere rises?
 
Exercise 3: Enzymes and pH
Observations  

Data Table 3: Glucose in wells a-d indicates enzyme vital-force at manifold pH levels
Well pH Concentration of Glucose
a 3.5 
b 5.0 
c 6.8 
d 11.0 

Questions  
A.  Graph the axioms placing glucose concentrations on the y-axis and the pH values on the x-axis.
B.   What was the commodities of pH on the enzyme lactase? Is this penny for all enzymes?