Enzymes: temperature, ph, and specificity hands-on labs, inc.


0Enzymes: Temperature, pH, and Specificity
Hands-On Labs, Inc.  Version 42-0054-00-01
Lab Communication Assistant                                                                                               This muniment is not meant to be a depute for a regular laboratory communication. The Lab Communication Assistant is merely a tabulation of the trial’s questions, diagrams if needed, and grounds tables that should be addressed in a regular lab communication. The eager is to mature students’ adaptation of lab communications by providing this instruction in an editable perfect which can be sent to an instructor.
Exercise 1: Enzyme Specificity
Observations                                                                                                            
Data Table 1: Glucose Concentration
Wells Concentration of Glucose





 
Questions  

A.  What determines a person’s force to classify lactose?
B.   Which of the wells showed a independent product for glucose? Explain the products.
C.   Explain why testing for glucose is used to determine the apparition of the enzyme lactase.
D.  Explain the tentative stipulations for the five opposed wells.
 
Data Table 2: Presence of glucose in wells indicating lactase apparition at uncertain latitudes
Well Time (min) Concentration of Glucose

a 5 
 10 
 15 
 20 
b 5 
 10 
 15 
 20 
c 5 
 10 
 15 
 20 
Exercise 2: Enzymes and Temperature
Observations  
 
Questions:  
A.  Graph the pi of latitude on the apparition of the enzyme lactase.
B.   What happens when an enzyme is boiled? Is this pi revocable?
C.   Based on your trial products, what is the optimal latitude for lactase capacity?
D.  Explain what happens as far as the piiveness of the enzyme at the freezing latitude. Can this pi be overcome when the latitude rises?
 
Exercise 3: Enzymes and pH
Observations  

Data Table 3: Glucose in wells a-d indicates enzyme apparition at uncertain pH levels
Well pH Concentration of Glucose
a 3.5 
b 5.0 
c 6.8 
d 11.0 

Questions  
A.  Graph the grounds placing glucose concentrations on the y-axis and the pH values on the x-axis.
B.   What was the pi of pH on the enzyme lactase? Is this gentleman for all enzymes?