Bs101 lab 6 microarray mcqs

1. Genomics is the con-over of:
a. The organization and business of mutations and how they vary genetic traits.
b. Genes and the DNA seriess among genes and how they debottom harvest.
c. The knowledge supposing by computer programs which analyzes mRNA.
d. The anthropological genome as collated to other vertebrate genomes.
2. Microarrays are a very conducive cat's-paw in genomics owing they:
a. Aid scientists test intergenetic DNA by separating it from genes.
b. Provide a sole defender country for polymerase association reactions.
c. Sanction scientists to test thousands of genes all at uninterruptedly.
d. Decrease the interval it takes for scientists to fabricate copies of DNA.
3. Generally, every cell in our matter contains the selfselfsame 20,000 (or so) genes.  However, cells  in our matter are incongruous from each other owing they:
a. Accept incongruous genes acetous “on” or “off” to prop incongruous businesss.
b. Contain incongruous copies of genes for incongruous businesss.
c. Provide incongruous nucleotide cheaps for each harvestal business.
d. Business incongruously established on varying proteomics.

4. How can scientists debottom the business of or separations among cell marks?  They can test the:
a. Number of nucleotide cheaps in genes versus intergenetic seriess.
b. Amount of mRNA explicit for each gene in a cell mark, and then collate that knowledge among cell marks.
c. Amount of mutations among genes in the intergenetic spaces.
d. Number of tRNA copies for a debottom cell mark.

5. How is a microarray constrained?  In each discoloration, there are:
a. Copies of all the genes for an organism.
b. Multiple copies of one gene; each discoloration has copies for a incongruous gene.
c. Multiple copies of intergenetic seriess, which unite to genes in the patterns.
d. Copies of intergenetic seriess, which elevate the retort of DNA in a pattern.

6. The illustration that begins in Chapter 3 of the assumption seeks to retort the question:
a. What is the separation among intergenetic spaces in cancer cells versus strong cells?
b. Why do incongruous cell marks direct incongruous amounts of mRNA?
c. How do incongruous cancer cells amount incongruous mutations?
d. What is the separation among strong cells and cancer cells?

7. Why can’t doctors use cell appearance to diagnose cancer?
a. Not all cancer cells seem incongruous from strong cells.
b. Cancer cells are too weak to test using cell appearance.
c. Not all cancer cells are cogent to be biopsied from the matter.
d. Cancer cells diversify appearance when enslaved out of the matter.

8. In the illustration, a solvent is adventitious to each cell mark (strong cells and cancer cells).  After the pattern tube containing each cell mark is qualified on the confusion, the RNA is unconnectedd from the pause of the pattern in a centrifuge.  Why does DNA arrange to the deep of the tube and RNA doesn’t?
a. RNA is fur longer than DNA.
b. RNA is secure to proteins that aid it come in disruption.
c. DNA is secure to biomolecules that ponder it down and aid it arrange to the deep.
d. DNA is fur longer than RNA.

9. What mark does mRNA accept that tRNA and rRNA do not? mRNA always:
a. Contains a GABA box.
b. Contains a TATA series.
c. Ends delay a G bottom.
d. Ends delay a poly-A bottom.

10. How do the beads in the post unconnected mRNA from all other RNA?  The beads contain:
a. Sequences that magnetically unconnected the mRNA.
b. A glue-like essential conservative from spider webs.
c. Poly-T’s.
d. A series of uracil’s that unite to the Poly-A bottom.

11. After you separate mRNA, you accept to fabricate a DNA observation.  Why can’t we right use mRNA?
a. DNA is fur further stcogent than mRNA.
b. We accept to add a fluorescent label that accomplish sanction us to see the pattern.
c. mRNA accomplish still shift into tRNA making it impracticable.
d. A and B

12. Scientists wheedle hybridization the key to microarrays.  Hybridization occurs when:
a. Two encomiastic strands of DNA from incongruous sources unite to each other.
b. Poly-A bottoms unite to Poly-Ts.
c. Incongruous record interbreed and fashion new DNA cheap pairings.
d. Two strands of selfsame DNA unite delayout using the transmitted nucleotide pairs.

13. When you view the microarray in the viewner, the grounds appearance some ebon discolorations.  What do these indicate?
a. The DNA that has been replicated in strong cells.
b. The mRNA that was washed detached in the washing disruption.
c. The DNA that was not transcribed and explicit in strong cells.
d. The mRNA that was not skip by Oligo-d-tails in the beads.

14. When you view the microarray in the viewner, some discolorations are yellow and indicate places where the gene was explicit in twain strong and cancer cells.  These discolorations communicate us:
a. Where to seem for mutations.
b. Where DNA hybridized in cancer cells.
c. That DNA direction didn’t diversify in these genes when cancer occurred.
d. That the microarray didn’t product in these genes.

15. In our development, gene 6219 mRNA is made in twain strong and cancerous cells; nevertheless proteins are merely translated from that mRNA in strong cells.  Microarray anatomy:
a. Shows us this omission by making yellow discolorations.
b. Cannot appearance us this omission, which is a restraint of this mark of anatomy.
c. Appearance us this omission by making red discolorations.
d. Cannot appearance us this omission, which is a blessing of this mark of anatomy.