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UMUC Biology 102/103

Lab 4: Enzymes

 

INSTRUCTIONS:

 

 

 

·         On your own and externally aid, adequate this Lab 4Answer Sheet electronically and acquiesce it via the Assignments Folder by the age listed intheCourse Schedule (below Syllabus).

 

·         To spend your laboratory exercises, use the Laboratory Manual located below Course Content. Read the preliminary and the directions for each exercise/exemplification carefully anteriorly completing the exercises/experiments and echoing the questions.

 

·         Save your Lab 4Answer Sheet in the subjoined format:  LastName_Lab4 (e.g., Smith_Lab4).

 

·         You should acquiesce your instrument as a Word (.doc or .docx) or Rich Text Format (.rtf) file for best compatibility.

 

 

 

 

Pre-Lab Questions

 

1.    How could you proof to see if an enzyme was adequately saturated during an exemplification?

 

 

 

 

 

2.    List three provisions that would remodel the ardor of an enzyme. Be biased delay your exposition.

 

 

 

 

 

 

 

3.       Take a observe encircling your family and warrant familyhold products that production by instrument of an enzyme. Call the products, and betoken how you perceive they production delay an enzyme.

 

 

 

 

Experiment 1: Enzymes In Food

 

Data Tables and Post-Lab Assessment

 

Table 1: Substance vs. Starch Presence

 

 

Substance

 

Resulting Color

 

Presence of Starch?

 

Is Amylase offer?

 

Positive Control: Starch

 

 

 

 

 

Negative Control: _____

Student must select

 

 

 

 

Food Product: ______

Student must select

 

 

 

 

Food Product: ______

Student must select

 

 

 

 

Saliva

 

 

 

 

 

 

 

Take a draw of your results. Comprise a still n ess delay your call and age on an refutation card in the draw. Insert draw here:

 

 

 

 

 

Post-Lab Questions

 

1.    What were your restrains for this exemplification? What did they conduct? Why was saliva moderate in this exemplification?

 

 

 

 

 

 

 

2.    What is the operation of amylase? What does amylase do to starch?

 

 

 

 

 

 

 

3.    Which of the foods that you proofed compriseed amylase? Which did not? What exemplificational averment assistances your assertion?

 

 

 

 

 

4.    Saliva does not comprise amylase until babies are two months old. How could this move an infant’s studyive requirements?

 

 

 

 

 

 

 

5.    There is another studyive enzyme (other than salivary amylase) that is secreted by the salivary glands. Research to enumerate what this enzyme is determined. What substrate does it act on? Where in the substantiality does it beseem activated, and why?

 

 

 

 

 

 

 

6.    Digestive enzymes in the gut comprise proteases, which study proteins. Why don’t these enzymes study the stomach and mean interior, which are imperfectly tight of protein?

 

 

Experiment 2: Effect of Sky on Enzyme Activity

 

Data Tables and Post-Lab Assessment

 

Table 2: Balloon Circumference vs. Temperature

 

Tube

Temperature (°C)

Balloon Circumference (Uninflated; cm)

Balloon Circumference (Inflated; cm)

1 - (Cold)

 

 

 

2 - (RT)

 

 

 

3 - (Hot)

 

 

 

 

 

 

Take a draw of your results. Comprise a still n ess delay your call and age on an refutation card in the draw. Insert draw here:

 

 

 

 

 

 

 

Post-Lab Questions

 

1.    What reaction is being catalyzed in this exemplification?

 

 

 

 

 

2.    What is the call of the enzyme in this exemplification? What is the substrate?

 

 

 

 

 

3.    What is the recalcitrant changeable in this exemplification? What is the trusting changeable?

 

 

 

 

 

 

 

4.    How does the sky move enzyme operation? Use averment from your postulates to assistance your defense.

 

 

 

 

 

5.    Draw a graph of balloon bisection vs. sky. What is the correspondence?

 

 

 

 

 

6.    Is there a privative restrain in this exemplification? If yes, warrant the restrain. If no, propose how you could re-examine the exemplification to comprise a privative restrain.

 

 

 

 

 

 

 

7.    In open, how would an growth in substrate remodel enzyme ardor? Draw a graph to explain this relationship.

 

 

 

 

 

8.    Design an exemplification to enumerate the optimal sky for enzyme operation, adequate delay restrains. Where would you invent the enzymes for this exemplification? What substrate would you use?