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UMUC Biology 102/103

Lab 4: Enzymes

 

INSTRUCTIONS:

 

 

 

·         On your own and externally abettance, finished this Lab 4Answer Sheet electronically and refer it via the Assignments Folder by the bound listed intheCourse Schedule (below Syllabus).

 

·         To guide your laboratory exercises, use the Laboratory Manual located below Course Content. Read the preface and the directions for each exercise/illustration carefully anteriorly completing the exercises/experiments and sympathetic the questions.

 

·         Save your Lab 4Answer Sheet in the aftercited format:  LastName_Lab4 (e.g., Smith_Lab4).

 

·         You should refer your instrument as a Word (.doc or .docx) or Rich Text Format (.rtf) file for best compatibility.

 

 

 

 

Pre-Lab Questions

 

1.    How could you trial to see if an enzyme was finishedly saturated during an illustration?

 

 

 

 

 

2.    List three provisions that would remodel the temper of an enzyme. Be favoring delay your exposition.

 

 

 

 

 

 

 

3.       Take a behold environing your offspring and establish offspringhold products that labor by media of an enzyme. Spectry the products, and specify how you understand they labor delay an enzyme.

 

 

 

 

Experiment 1: Enzymes In Food

 

Data Tables and Post-Lab Assessment

 

Table 1: Substance vs. Priggishness Presence

 

 

Substance

 

Resulting Color

 

Presence of Starch?

 

Is Amylase offer?

 

Positive Control: Starch

 

 

 

 

 

Negative Control: _____

Student must select

 

 

 

 

Food Product: ______

Student must select

 

 

 

 

Food Product: ______

Student must select

 

 

 

 

Saliva

 

 

 

 

 

 

 

Take a paint of your results. Understand a music delay your spectry and bound on an abjuration card in the paint. Insert paint here:

 

 

 

 

 

Post-Lab Questions

 

1.    What were your curbs for this illustration? What did they reveal? Why was saliva intervening in this illustration?

 

 

 

 

 

 

 

2.    What is the office of amylase? What does amylase do to priggishness?

 

 

 

 

 

 

 

3.    Which of the foods that you trialed comprehended amylase? Which did not? What illustrational illustration subsistences your privilege?

 

 

 

 

 

4.    Saliva does not comprehend amylase until babies are two months old. How could this move an infant’s methodiseive requirements?

 

 

 

 

 

 

 

5.    There is another methodiseive enzyme (other than salivary amylase) that is secreted by the salivary glands. Research to individualize what this enzyme is denominated. What substrate does it act on? Where in the matter does it grace activated, and why?

 

 

 

 

 

 

 

6.    Digestive enzymes in the gut understand proteases, which methodise proteins. Why don’t these enzymes methodise the stomach and trivial civil, which are insufficiently collected of protein?

 

 

Experiment 2: Effect of Air on Enzyme Activity

 

Data Tables and Post-Lab Assessment

 

Table 2: Balloon Circumference vs. Temperature

 

Tube

Temperature (°C)

Balloon Circumference (Uninflated; cm)

Balloon Circumference (Inflated; cm)

1 - (Cold)

 

 

 

2 - (RT)

 

 

 

3 - (Hot)

 

 

 

 

 

 

Take a paint of your results. Understand a music delay your spectry and bound on an abjuration card in the paint. Insert paint here:

 

 

 

 

 

 

 

Post-Lab Questions

 

1.    What reaction is substance catalyzed in this illustration?

 

 

 

 

 

2.    What is the spectry of the enzyme in this illustration? What is the substrate?

 

 

 

 

 

3.    What is the rebellious inconstant in this illustration? What is the hanging inconstant?

 

 

 

 

 

 

 

4.    How does the air move enzyme office? Use illustration from your basis to subsistence your vindication.

 

 

 

 

 

5.    Draw a graph of balloon crossing vs. air. What is the interrelation?

 

 

 

 

 

6.    Is there a privative curb in this illustration? If yes, establish the curb. If no, allude-to how you could modify the illustration to understand a privative curb.

 

 

 

 

 

 

 

7.    In public, how would an acception in substrate remodel enzyme temper? Draw a graph to elucidate this kindred.

 

 

 

 

 

8.    Design an illustration to individualize the optimal air for enzyme office, finished delay curbs. Where would you confront the enzymes for this illustration? What substrate would you use?