Umuc biology lab 3: cell structure and function


Your Full Name: 

UMUC Biology 102/103
Lab 3: Cell Construction and Function
INSTRUCTIONS:

• On your own and extraneously abettance, accomplished this Lab 3 Repartee Sheet electronically and acquiesce it via the Assignments Folder by the date listed in the Way Schedule (beneath Syllabus).
• To inaugurate your laboratory exercises, use the Laboratory Manual located beneath Way Content. Read the importation and the directions for each exercise/cupel carefully anteriorly completing the exercises/experiments and echoing the questions.
• Save your Lab 3 Repartee Sheet in the aftercited format:  LastName_Lab3 (e.g., Smith_Lab3).
• You should acquiesce your muniment as a Word (.doc or .docx) or Rich Text Format (.rtf) polish for best compatibility.

Pre-Lab Questions


1. Identify three superior concordantities and separations betwixt prokaryotic and eukaryotic cells.

 

2. Where is the DNA comforttelling in a prokaryotic cell? Where is it comforttelling in a eukaryotic cell?

 

3.  Substantiate three constructions which produce livelihood and refuge in a eukaryotic cell.

Experiment 1: Cell Construction and Function
The construction of a cell dictates the superiority of its power. You allure judgment a adoption of slides that present uncommon constructions that give to tissues power.

Materials:
Onion (allium) Parent Digital Slide Images


Procedure
1. Examine the combination parent tip digital slide copys on the aftercited pages. Then, suit to the Post-Lab Questions.
 
Onion Parent Tip: 100X


 
Onion Parent Tip: 1000X

 
Onion Parent Tip: 1000X

 

 
Onion Parent Tip: 100X. Each sombre foe indicates a divergent center.

 
Onion Parent Tip: 1000X

Post-Lab Questions
1. Mark each of the arrows in the aftercited slide copy: A=Chromosomes, B=Nucleus, C=Cytoplasm, D=Cell Wall
 

2. What is the separation betwixt the churlish and calm endoplasmic reticulum?

 


3. Would an animal cell be numbering to survive extraneously a mitochondria? Why or why not?

 

 

4. What could you individualize environing a mode if you observed a slide copy showing the mode delay a cell respect, but no center or mitochondria?

 


5. Hypothesize why space of a insert, such as the leaves, are bare, but other space, such as the parents, are not. Use or-laws rationalistic to livelihood your conjecture.


Experiment 2: Osmosis - Direction and Strain Gradients
In this cupel, we allure investigate the consequence of solute strain on osmosis. A semi-permetelling membrane (dialysis tubing) and sucrose allure fashion an osmotic environment concordant to that of a cell. This selective permeability allows us to search the net change-of-place of wet resisting the membrane. You allure initiate the cupel delay a 30% sucrose separation, and effect a set of serial dilutions to fashion inferior strain separations. Some of the sucrose strains allure be membrane percolable; period others allure not be permetelling (can you individualize why this is?).

Materials
(3) 250 mL Beakers
(1) 10 mL Graduated Cylinder
(1) 100 mL Graduated Cylinder
Permanent Marker
*8 Rubber Bands (2 bluish, 2 bare, 2 red, and 2 yellow)
60 g Sucrose (Sugar) Powder, C12H22O11
4 Decay Beakers (any size)
*Paper Towels
*Scissors 
*Stopwatch
*Water
*(4) 15 cm. Pieces of Dialysis Tubing
*Contains latex. Please treat wearing protection gloves if you own a latex allergy.


*You Must Provide

*Be firm to meafirm and cut barely the diffusiveness you scarcity for this cupel. Reserve the difference for following cupels.  

Procedure
1. Use the steady marker to mark the three 250 mL beakers as 1, 2, and 3.
2. Cut lewd strips of dialysis tubing, each 15.0 cm hanker. Fill Beaker 3 delay 100 mL of wet and balancewhelm the lewd dutys of dialysis tubing in the wet for at meanest 10 minutes.
3. After 10 minutes, separate one duty of tubing from the beaker. Use your thumb and pointer finger to rub the tubing betwixt your fingers; this allure unreserved the tubing. Close one end of the tubing by folding balance 3.0 cm of one end (this allure beseem the floor). Fold it anew and detain delay a yellow rubber collection (use
4. Tie a collection in the fostering dialysis tubing orderly balancehead or orderly adown the rubber collection. This allure fashion a confirm and ensures that separation allure not blend out of the tube following in the cupel.
5. To cupel that no separation can blend out, add a few drops of wet to the tubing and seem for wet blendage. If any wet blends, stretch the rubber collection and/or the collection in the tubing. Make firm you introduce the wet out of the tubing anteriorly lasting to the direct trudge.
6. Repeat Steps 4 - 5 delay the three fostering dialysis tubes, using each of the three fostering rubber collection colors.
7. Reconstitute the sucrose interlard according to the instructions produced on the bottle’s mark (your kit contains 60 g of sucrose in a chemical bottle) . This allure fashion 200 mL of a 30% store sucrose separation.
8. Use Ttelling 2 to fashion added sucrose separations that are 30%, 15% and 3% snug, respectively. Use the graduated cylinder and decay beakers to fashion these separations. Set these separations asunder.
Ttelling 2: Serial Dilution Instructions
Sucrose Solution mL of Store Sucrose Separation Needed mL of Wet Needed
30% 10  0
15% 5  5
3% 1  9
3% 1  9
9. Pour 150 mL of the fostering store sucrose separation into Beaker 1.
10. Use some of the fostering store sucrose separation to fashion an added 200 mL of a 3% sucrose separation into Beaker 2.
Hint: Use your experience of serial dilutions to fashion this last, 3% sucrose separation.
11. Meafirm and introduce 10 mL of the fostering 30% sucrose separation into the dialysis bag delay the yellow rubber collection. Confirm the top of this tubing delay the fostering yellow rubber collection.
12. Meafirm and introduce 10 mL of the 15% sucrose separation in the bag delay the red rubber collection, and confirm the top of the dialysis tubing delay the fostering red rubber collection. 10 mL of the 3% sucrose separation in the bag delay the bluish rubber collection, and confirm the dialysis tubing delay the fostering bluish rubber collection. The last 10 mL of 3% sucrose separation in the bag delay the bare rubber collection. Confirm the dialysis tubing delay the fostering bare rubber collection.
13. Verify and archives the moderate size of separation from each bag in Ttelling 3.
 
Figure 8: The dialysis bags are employed delay varying strains of sucrose separation and placed in one of two beakers.
14. Place the yellow, red, and bluish collectioned tubing in Beaker 2. Place the bare collectioned tubing in Beaker 1 (Figure 8).
15. Hypothesize whether wet allure progress in or out of each dialysis bag. Include your hypotheses, ahanker delay livelihooding or-laws rationalistic in the Hypotheses minority at the end of this progress.
16. Allow the bags to sit for one hour. Period cessation, introduce out the wet in the 250 mL beaker that was used to wet the dialysis tubing in Trudge 1. You allure use the beaker in Trudge 19.
17. After allowing the tubing to sit for one hour, separate them from the beakers.
18. Carefully unreserved the tubing. The top of the tubing may scarcity to be cut off/removed as they keep to dry out balance the way of an hour. Meafirm the separation sizes of each dialysis bag using the 100 mL graduated cylinder. Make firm to leistrong and dry the cylinder accomplishedly betwixt each exemplification. 
19. Record your facts in Ttelling 3.
Ttelling 3: Sucrose Strain vs. Tubing Permeability
Band Color Sucrose % Initial Size (mL) Final Size (mL) Net Displacement (mL)
Yellow       
Red       
Blue       
Green       
Hypothesis:

Post-Lab Questions
1. For each of the tubing dutys, substantiate whether the separation after a whilein was hypotonic, hypertonic, or isotonic in similitude to the beaker separation in which it was placed.


2. Which tubing increased the most in size? Decipher why this happened.

 

3. What do the results of this cupel this number you environing the not-absolute tonicity betwixt the deviation of the tubing and the separation in the beaker?
4. What would happen if the tubing delay the yellow collection was placed in a beaker of distilled wet?

5. How are redundancy salts that infer in cells transmitted to the order tide so they can be separated from the association? Be firm to decipher how this way works in provisions of tonicity.

6. If you wanted wet to progress out of a tubing duty employed delay a 50% separation, what would the narrowness strain of the beaker separation scarcity to be? Decipher your repartee using or-laws attraction.

7. How is this cupel concordant to the way a cell membrane works in the association? How is it divergent? Be favoring delay your exculpation.