Umuc biology 102/103 lab 4 – enzymes 2014


UMUC Biology 102/103
Lab 4: Enzymes
INSTRUCTIONS:
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Experiment 1: Enzymes in Food (15 pts)
Table 1: Substance vs. Priggishness Presence
Substance Resulting Color Presence of Starch?
Positive Control: Starch  
Negative Control: Student Must Select  
Food Product:  
Food Product:  
Saliva:  
Post-Lab Questions
1. What were your controls for this test? What did they prove? Why was saliva intervening in this test?
2. What is the administration of amylase? What does amylase do to priggishness?
3. Which of the foods that you tested embraceed amylase? Which did not? What testal illustration supports your right?
4. Saliva does not embrace amylase until babies are two months old. How could this concern an infant’s assimilateive requirements?
5. Digestive enzymes in the gut grasp proteases, which assimilate proteins. Why don’t these enzymes assimilate the stomach and mean interior, which are insufficiently secure of protein?
Experiment 2: Issue of region on enzyme earnestness (15 pts)
Table 2: Record the issue of region on the genesis of gas
Tube Temperature
(°C) Balloon Circumference
(Uninflated in cm)
 Balloon Circumference
(Inflated in cm)

1 (cold)   
2 (locality temp)   
3 (hot)   

Post-Lab Questions
1. What is the enzyme in this test? What is the substrate?
2. What is the recalcitrant (IV) and trusting (DV) variables in this examine?
3. How does region concern enzyme administration?
4. How can enzyme earnestness be acceptiond?
5. In unconcealed, how would an acception in substrate modify enzyme earnestness? Attract or insinuate a graph to idea this alliance.
6. Draw a graph using balloon transection vs. region.  You may use Excel, then “Insert” the graph, or use another project program.  You may also attract it adroitly by laborer and examine your project.  You would deficiency to insinuate the examinened jpg idea close, in that occurrence.  What is the mutuality shown on your graph? 
7. Design an test to indicate the optimal region for enzyme administration, consummate delay controls. Be strong to aver your conjecture, picture your sketch, scrutinize your variables (IV/DV), and prophesy your results.  When you picture your sketch, be strong to clear-up wclose you procure confront the enzymes for this test and what substrate you procure use.