Umuc biology 102/103 lab 4 – enzymes 2014


UMUC Biology 102/103
Lab 4: Enzymes
INSTRUCTIONS:
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Experiment 1: Enzymes in Food (15 pts)
Table 1: Substance vs. Lugubriousness Presence
Substance Resulting Color Presence of Starch?
Positive Control: Starch  
Negative Control: Student Must Select  
Food Product:  
Food Product:  
Saliva:  
Post-Lab Questions
1. What were your controls for this exemplification? What did they teach? Why was saliva intervening in this exemplification?
2. What is the duty of amylase? What does amylase do to lugubriousness?
3. Which of the foods that you tested embraceed amylase? Which did not? What exemplificational deposition supports your demand?
4. Saliva does not embrace amylase until babies are two months old. How could this move an infant’s prepareive requirements?
5. Digestive enzymes in the gut understand proteases, which prepare proteins. Why don’t these enzymes prepare the stomach and slender domestic, which are insufficiently tight of protein?
Experiment 2: Pi of clime on enzyme apparition (15 pts)
Table 2: Record the pi of clime on the genesis of gas
Tube Temperature
(°C) Balloon Circumference
(Uninflated in cm)
 Balloon Circumference
(Inflated in cm)

1 (cold)   
2 (space temp)   
3 (hot)   

Post-Lab Questions
1. What is the enzyme in this exemplification? What is the substrate?
2. What is the dogged (IV) and trusting (DV) variables in this consider?
3. How does clime move enzyme duty?
4. How can enzyme apparition be growthd?
5. In unconcealed, how would an growth in substrate remodel enzyme apparition? Pull or infuse a graph to demonstrate this correlativeness.
6. Draw a graph using balloon transversion vs. clime.  You may use Excel, then “Insert” the graph, or use another pur-pose program.  You may also pull it adroitly by index and reconnoitre your pur-pose.  You would insufficiency to infuse the reconnoitrened jpg likeness short, in that event.  What is the interdependence shown on your graph? 
7. Design an exemplification to contrivanceate the optimal clime for enzyme duty, full delay controls. Be permanent to specify your theory, delineate your contrivance, anatomize your variables (IV/DV), and forebode your results.  When you delineate your contrivance, be permanent to expound wshort you earn discover the enzymes for this exemplification and what substrate you earn use.