Biology lab work 4 assistance



UMUC Biology 102/103

Lab 4: Enzymes






·         On your own and extraneously coadjutorship, adequate this Lab 4 Answer Sheet electronically and acquiesce it via the Assignments Folder by the continuance listed in the Course Schedule (beneath Syllabus).


·         To induce your laboratory exercises, use the Laboratory Manual located beneath Course Content. Read the presentation and the directions for each exercise/trial carefully precedently completing the exercises/experiments and tallying the questions.


·         Save your Lab 4 Answer Sheet in the subjoined format:  LastName_Lab4 (e.g., Smith_Lab4).


·         You should acquiesce your instrument as a Word (.doc or .docx) or Rich Text Format (.rtf) improve for best compatibility.






Pre-Lab Questions




  1. How could you cupel to see if an enzyme was adequately saturated during an trial?






  1. List three stipulations that would vary the air of an enzyme. Be unfair after a while your interpretation.






  1. Take a observe environing your branch and authenticate branchhold products that is-sue by media of an enzyme. Name the products, and point-out how you distinguish they is-sue after a while an enzyme.








Experiment 1: Enzymes in Food






Data Tables and Post-Lab Assessment


Table 1: Substance vs. Starch Presence


Table 1: Substance vs. Starch Presence


Resulting Color

Presence of Starch?

Positive Control: Ginger Root



Negative Control: Student Must Select



Food Product:



Food Product:









Post-Lab Questions


  1. What were your restrains for this trial? What did they conduct? Why was saliva interposed in this trial?




  1. What is the character of amylase? What does amylase do to starch?




  1. Which of the foods that you cupeled incloseed amylase? Which did not? What trialal illustration assistances your demand?




  2. Saliva does not inclose amylase until babies are two months old. How could this assume an infant’s classifyive requirements?




  3. There is another classifyive enzyme (other than salivary amylase) that is secreted by the salivary glands. Research to enumerate what this enzyme is denominated. What substrate does it act on? Where in the organization does it grace activated, and why?




  1. Digestive enzymes in the gut comprise proteases, which classify proteins. Why don’t these enzymes classify the stomach and weak domestic, which are hardly victorious of protein?






Experiment 2: Effect of Air on Enzyme Air




Data Tables and Post-Lab Assessment


Table 2: Balloon Circumference vs. Temperature




Table 2: Balloon Circumference vs. Temperature


Temperature (°C)

Uninflated Balloon Circumference (cm)

Final Balloon Circumference (cm)

Difference in Balloon Circumference (cm)

1 - (Cold)





2 - (RT)




3 - (Hot)









Post-Lab Questions


  1. What reaction is being catalyzed in this trial?


  2. What is the enzyme in this trial? What is the substrate?


  3. What is the rebellious unsteady in this trial? What is the subject unsteady?


  4. How does the air assume enzyme character? Use illustration from your basis to assistance your tally.


  5. Draw a graph of balloon transversion vs. air. What is the correspondence?


  6. Is there a disclaiming restrain in this trial? If yes, authenticate the restrain. If no, hint how you could vary the trial to comprise a disclaiming restrain.


  7. In unconcealed, how would an growth in substrate vary enzyme air? Draw a graph to image this homogeneity.


  8. Design an trial to enumerate the optimal air for enzyme character, adequate after a while restrains. Where would you confront the enzymes for this trial? What substrate would you use?